At Antibody-Elisa you will find the principle and method of ELISA. Enzyme-linked immunosorbent assay (ELISA) is a method of target antigen (or antibody) capture in samples using a specific antibody (or antigen), and of target molecule detection/quantitation using an enzyme reaction with its substrate. Elisa Test Procedure an enzyme-linked immunosorbent assay is a test that detects and measures antibodies in your blood. This test can be used to determine if you have antibodies related to certain infectious conditions. Usually an ELISA test is used to diagnose: HIV, which causes AIDS. At Antibody-ELISA.com you will find all the information related to Elisa.

ELISA is a plate based assay technique which is used for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. An enzyme conjugated with an antibody reacts with colorless substrate to generate a colored product. Such substrate is called chromogenic substrate. A number of enzymes have been used for ELISA sample preparation such as alkaline phosphatase, horse radish peroxidase and beta galactosidase.

Among the different types of ELISA, Sandwich ELISA protocol detects antigen. In this technique, antibody is coated on the microtiter well. A sample containing antigen is added to the well and allowed to react with the antibody attached to the well, forming antigen-antibody complex. After the well is washed, a second enzyme-linked antibody specific for a different epitope on the antigen is added and allowed to react with the bound antigen. Then after unbound secondary antibody is removed by washing and finally substrate is added to the plate which is hydrolyzed by enzyme to form coloured products.

Sandwich ELISA has several advantages such as high specificity since two antibodies are used the antigen is specifically captured and detected. It is suitable for complex samples, since the antigen does not require purification prior to measurement. It offers flexibility and sensitivity, since both direct and indirect detection methods can be used. It is competitive ELISA as this test is used to measure the concentration of an antigen in a sample.

About Antibody Elisa:

Modern medicine recognizes the complications of treating infectious diseases. It is realized that early diagnosis of diseases is extremely important these days.