DEAE-Cellulose is used in ion exchange chromatography, protein chromatography and anion exchange media. DEAE cellulose has been used to study acute myocardial infarction. Techniques which are widely used in protein purification are salting-out by high concentrations of ammonium sulfate and ion-exchange chromatography on a column of a resin such as diethyl aminoethyl cellulose (DEAE-cellulose). In purification procedure, it is used to remove excess salt prior application of the protein mixture to the column. Generally column of DEAE-cellulose chromatography are applied in a solution that contains a high concentration of ammonium sulfate

SERVAYLT Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of consequent deprived species of average molecular weight distribution and comprise different pI-values. In agarose and polyacrylamide gels containing ampholytes, a linear pH-gradient will be built up when an electric field is applied – the ampholyte molecules carry a net charge and thus migrate in the electric field between the electrodes as long as they will reach the position of corresponding pI.

Good ampholyte mixtures comprise low molecular weight species of different pI-values which contribute to conductivity, an important criterion to yield reliable results and excellent reproducibility. The composition of the carrier ampholyte mixture is useful when complex samples are to be resolved featuring a pl-focus.

Benefits of SERVALYT Carrier

• SERVALTY Carrier Ampholytes is produced as per to the highest quality standards and are frequently tested for performance.
• Ensure fast staining and distaining times
• Clear background associated with very low unspecific binding of dyes and stains
• High solubility in trichloroacetic acid
• Virtually no interaction with metal ions

Widely used for research works, we at Bio Phoretics sell ready to use SERVALYT™ fractions are ready-to-use. Very acidic (pH 2-4) and very basic ampholytes (pH 7-9/9-11) may cause problems in polymerization. We advise to add 20 % SERVALYT 3-10 (Cat. No. 42940). Prior to casting, simply mix 8 ml of acidic or basic SERVALYT with 2 ml of wide range SERVALYT.

Generally Ampholyte fractions spanning 1 pH unit are recommended to preblending. Product usage and application All SERVALYT are suited to prepare any gel for isoelectric focusing made of polyacrylamide or agarose. The common working concentration is in the range of 3 % to 5 %. Technical grade SERVALYT carrier ampholyte pH 4-9 T is economical to use if preparative work is envisaged. SERVALYT™ ampholyte are also applicable to medium-free IEF systems, e. g. free flow electrophoresis and capillary electrophoresis
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