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Stable developments and their component proteins can be easily separated on the basis of their net charge by ion-exchange chromatography. DEAE – Diethyl Amino Ethyl Cellulose columns are widely used for protein purification. DEAE cellulose is an anionic resin which can be widely used to bind negatively charged proteins. Actually proteins can be eluted by altering either by increasing the salt concentration or by altering the pH.

DEAE - Diethyl Amino Ethyl Cellulose columns are used for Protein purification. DEAE cellulose chromatography is an anionic resin which can be used to bind -vely charged proteins.Proteins can be eluted by altering either by increasing the salt concentration or by altering the pH.Protein that have a low density of net positive charge will tend to emerge first, keep on by those having a higher charge density. Positively charged complexes or proteins can be separated on negatively charged carboxymethyl-cellulose (CM-cellulose) columns.

It won’t be wrong to say that DEAE is a weak ion exchange resin and hence the pH range at which it can be used is limited and thus charge density will vary.The diethylamino group of DEAE-cellulose carries a positive charge which play crucial role for the ion-binding properties of this resin. Effectively, negatively charge damino acids/proteins will interact with the diethylamino group while positively charged amino acids/proteins will be eluted.

When a fractionation with ammonium sulphate precedes ion-exchange chromatography in a purification procedure often help to remove excess salt prior application of the protein mixture to the column. Basically it is done to ensure that the ionic strength of the solution is sufficiently low for absorption of at least some of the proteins in the mixture.

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View source: https://www.issuewire.com/use-of-deae-cellulose-chromatography-in-research-field-1705261314764400