In the field of biomedical research, the immunostaining and immunoassay applications work on a simple principle of using the best antibodies. The quality of this rabbit antibody production is particularly important since only then can it go through the various research programs. Issues like cross-reactivity, batch to batch variability, and the use of antibodies in the untested applications have all corrupted years of research programs. To avoid such issues, the only safe way is to select the right antibodies so that you do not disrupt your years of research with devastating effects. The following are some of the best tips that will help you in the right antibody selection.

Find an antibody that is suitable for the reaction: Different antibodies are needed for different receptor reactions. Therefore, you must find the right type of it that is compatible enough with your research. Also, the product datasheet supplied with the antibody should detail all the applications for which it has been tested. This is the job of the company producing the antibodies for research purposes. Also, the applications for which it has been produced and it has produced the expect the pattern of staining should also be included.

Choose a compatible secondary antibody: Secondary antibody is specifically raised against the host species of the primary antibody. They are available in various formats. Therefore, it is very important that you select the right secondary antibody according to their application. It is pivotal for the rigorous validation and to ensure that no cross-reactivity reaction takes place.

Refer to the literature: The more you read about the process, the more you will gain knowledge about it. Therefore, refer to as much of literature as you can to determine which applications and experimental conditions have been successful with which antibody product. Also, read full details of references that cite the use of its antibodies. Read about the sheep endothelin A receptor as well.

Examine the protocols for any results: All the protocols related with immunostaining require careful optimization, and consideration of several factors including the nature of blocking butter, antibody diluents, and wash solutions.

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